International Journal of Applied Microbiology and Biotechnology Research
ISSN: 2053-1818
Vol. 1(4), pp. 20-25, December 2013
doi.org/10.33500/ijambr.2013.01.004



Evaluation of methods for the extraction and purification of DNA of cultured Lactobacillus colony isolated from dairy products

Thikra A. Abed*

Department of Biology-Biotechnology, College of Sciences, Babylon University, Babylon, Iraq.

*To whom correspondence should be addressed. E-mail: alhussainybio@yahoo.com.

Received 18 August, 2013; Received in revised form 25 October, 2013; Accepted 02 November, 2013.

Abstract


Key words:
Lactobacillus, DNA extraction, Polymerase chain reaction (PCR), DNA purity.


Lactic acid bacteria are very significant to human health due to the production of some antimicrobial substances and ability to inhibit pathogenic bacteria. Furthermore, the bacteria are also used as starter culture in the production of various foods. The aim of this study was to evaluate five methods for the extraction and purification of DNA from 6 isolates of Lactobacillus colony, isolated from dairy product. The methods were: Modified Pospiech and Neumann, Martin–Platero, Phenol–chloroform, Wizard genomic DNA purification kit and Wizard genomic DNA purification kit with modifications. The results obtained in the study confirmed that extracted genomic DNA using Wizard genomic DNA purification kit with modifications was superior to other methods because it produced a higher DNA yield with the highest purity. Traditional polymerase chain reaction (PCR) was optimized to detect Lactobacillus spp. The molecular based culture-dependent methods depend on several factors including best DNA extraction and specific primer set to achieve PCR assay.

2013 BluePen Journals Ltd.







© 2013-2021 - BluePen Journals | Terms | Privacy policy | Advert | Help |